Lactate Dehydrogenase A anticorps (HRP)

N° du produit ABIN5596934

N° du produit (Fournisseur): 200-1373-0100

L’anticorps Chèvre Polyclonal anti-Lactate Dehydrogenase A a été validé pour WB, IHC, ELISA, IP et IF. Il convient pour détecter Lactate Dehydrogenase A dans des échantillons de Lapin.

Aperçu rapide pour Lactate Dehydrogenase A anticorps (HRP) (ABIN5596934)

Antigène: Lactate Dehydrogenase A (LDHA)

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Lactate Dehydrogenase A est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Détail du produit anti-Lactate Dehydrogenase A anticorps (HRP)

N° du produit (Fournisseur): 200-1373-0100

Fournisseur: Rockland

Fonction: Lactate Dehydrogenase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].

Attributs du produit: Synonyms: goat anti-Lactate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M

Purification: Anti-Lactate Dehydrogenase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Anti-Lactate Dehydrogenase Antibody was produced by repeated immunizations with rabbit muscle Lactate Dehydrogenase full length protein.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Lactate Dehydrogenase peroxidase Antibody has been tested in ELISA, western blot, and immunofluorescence. This product is suitable for IP and IHC. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:6,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:3,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Lactate Dehydrogenase A

Antigène: Lactate Dehydrogenase A (LDHA)

Autre désignation: LDHA

Sujet: Background: Anti-Lactate Dehydrogenase Antibody detects Lactate Dehydrogenase. Lactate dehydrogenases exist in four distinct enzyme classes. Two of them are cytochrome c-dependent enzymes, each acting on either D-lactate or L-lactate. The other two are NAD(P)-dependent enzymes, each acting on either D-lactate or L-lactate. Lactate dehydrogenase catalyzes the interconversion of pyruvate and lactate. Anti-Lactate Dehydrogenase Antibody is ideal for investigators involved in Cell Signaling, Immunology, Neuroscience and Signal Transduction research.

ID gène: 100009107, 126050

UniProt: P13491

Pathways: L'effet Warburg